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ORIGINAL ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 2  |  Page : 58-62  

Mandibular ramus: An indicator for sex determination - A digital radiographic study


Department of Oral Medicine and Radiology, M. R. Ambedkar Dental College and Hospital, Bangalore, Karnataka, India

Date of Web Publication1-Apr-2013

Correspondence Address:
Archana Markande
Department of Oral Medicine and Radiology, M. R. Ambedkar Dental College and Hospital, Bangalore, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-1475.109885

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   Abstract 

Background: The identification of skeletal remains is of paramount importance in medico-legal investigations. The skeletal components most often investigated for gender determination are the pelvis and skull, with the mandible being a practical element to analyze sexual dimorphism in the fragmented bones. Presence of a dense layer of compact bone makes it very durable and well preserved than many other bones. Mandibular ramus can be used to differentiate between sexes and it also expresses strong univariate sexual dimorphism. When skeleton sex determination is considered, metric analyses on the radiographs are often found to be of superior value owing to their objectivity, accuracy, and reproducibility. Aims and Objectives: (1) To measure, compare, and evaluate the various measurements of mandibular ramus as observed on orthopantomographs. (2) To assess the usefulness of mandibular ramus as an aid in sex determination. Materials and Methods: A retrospective study was conducted using orthopantomographs of 50 males and 50 females, which were taken using Kodak 8000C Digital Panoramic and Cephalometric System (73 kVp, 12 mA, 13.9 s). Mandibular ramus measurements were carried out using Master View 3.0 software. The measurements of the mandibular ramus were subjected to discriminant function analysis. Results: We observed each variable of the mandibular ramus to be a significant predictor in classifying a given sample (P < 0.001). Conclusion: This study on mandibular ramus measurements using orthopantomograph shows strong evidence suggesting that the ramus can be used for gender determination for forensic analysis.

Keywords: Discriminant function analysis, mandibular ramus, orthopantomograph, sexual dimorphism


How to cite this article:
Indira AP, Markande A, David MP. Mandibular ramus: An indicator for sex determination - A digital radiographic study. J Forensic Dent Sci 2012;4:58-62

How to cite this URL:
Indira AP, Markande A, David MP. Mandibular ramus: An indicator for sex determination - A digital radiographic study. J Forensic Dent Sci [serial online] 2012 [cited 2017 Jan 18];4:58-62. Available from: http://www.jfds.org/text.asp?2012/4/2/58/109885


   Introduction Top


The identification of human skeletal remains is considered an initial step in forensic investigations and is crucial for further analysis. [1] In the adult skeleton, sex determination is usually the first step of the identification process as subsequent methods for age and stature estimation are sex dependent. The reliability of sex determination depends on the completeness of the remains and the degree of sexual dimorphism inherent in the population. [2] When the entire adult skeleton is available for analysis, sex can be determined up to 100% accuracy, but in cases of mass disasters where usually fragmented bones are found, sex determination with 100% accuracy is not possible and it depends largely on the available parts of skeleton. [1],[2]

As evident from the earlier studies, skull is the most dimorphic and easily sexed portion of skeleton after pelvis, providing accuracy up to 92%. [1] But in cases where intact skull is not found, mandible may play a vital role in sex determination as it is the most dimorphic, largest, and strongest bone of skull. [1],[2],[3],[4] Presence of a dense layer of compact bone makes it very durable, and hence remains well preserved than many other bones. Dimorphism in mandible is reflected in its shape and size. [1] Male bones are generally bigger and more robust than female bones. [2]

The relative development (size, strength, and angulation) of the muscles of mastication is known to influence the expression of mandibular dimorphism as masticatory forces exerted are different for males and females. [5] Humphrey et al. showed that the sites associated with the greatest morphological changes in size and remodeling during growth, mandibular condyle, and ramus in particular are generally the most sexually dimorphic. Measurements of the mandibular ramus tend to show higher sexual dimorphism, and differences between the sexes are generally more marked in the mandibular ramus than in the mandibular body. [6] Methods based on measurements and morphometry are accurate and can be used in determination of sex. [7]

Dentofacial radiography has become a routine procedure in the dental, medical, and hospital clinics. The radiographs are taken at different periods during the lifetime of large segments of the population. [8] Rotational panoramic radiography is widely used for obtaining a comprehensive overview of the maxillofacial complex. [9] In forensic anthropology, comparison of antemortem and postmortem radiographs is one of the cornerstones of positive identification of human remains. Antemortem orthopantomograms may be of great value in the identification of human remains. [10] Several studies have been conducted on dry adult mandibles for sex determination, [1],[4],[5],[6],[7] but a literature search did not reveal any studies with regard to measurements on ramus of the mandible using a digital panoramic radiograph.

Hence, this paper aims to evaluate the usefulness of mandibular ramus in sex discrimination in Bangalore population and propose the use of same in forensic analysis.


   Aims and Objectives Top


The present study was designed with the following aims and objectives:

  1. To measure, compare, and evaluate the various measurements of mandibular ramus as observed on digital orthopantomographs.
  2. To assess the usefulness of mandibular ramus as an aid in sex determination.

   Materials and Methods Top


A retrospective study was conducted using orthopantomographs of 50 males and 50 females of Bangalore population in the age group between 20 and 50 years. Ideal orthopantomographs of completely dentate patients were selected for the study. Pathological, fractured, developmental disturbances of the mandible, deformed and edentulous mandibles were excluded from the study. Radiographs taken by Kodak 8000C Digital Panoramic and Cephalometric System (73 kVp, 12 mA, 13.9 s) were used for the study. Since this study was conducted on radiographs stored in the system, ethical clearance was not applicable. Mandibular ramus measurements were carried out using Master View 3.0 software.

The following parameters were measured using mouse-driven method (by moving the mouse and drawing lines using chosen points on the digital panoramic radiograph) [Figure 1] and [Figure 2]:

  1. Maximum ramus breadth: The distance between the most anterior point on the mandibular ramus and a line connecting the most posterior point on the condyle and the angle of jaw. [1],[7]
  2. Minimum ramus breadth: Smallest anterior-posterior diameter of the ramus. [1]
  3. Condylar height⁄maximum ramus height: Height of the ramus of the mandible from the most superior point on the mandibular condyle to the tubercle, or most protruding portion of the inferior border of the ramus. [1]
  4. Projective height of ramus: Projective height of ramus between the highest point of the mandibular condyle and lower margin of the bone. [1]
  5. Coronoid height: Projective distance between coronion and lower wall of the bone. [1]
Figure 1: Diagram showing mandibular ramus measurements adapted from Saini et al[1]

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Figure 2: Measurements of mandibular ramus on orthopantomograph

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Statistical analysis

The data were analyzed using the discriminant procedure of the statistical package SPSS 13.0. Discriminant function analysis was used to determine variables that discriminate between male and female and is increasingly utilized for sex diagnosis from skeletal measurements.


   Results Top


Descriptive statistics of five mandibular ramus measurements and associated univariate F ratios for both sexes are shown in [Table 1]. We noticed that each variable was a significant predictor in classifying a given sample (P < 0.001). The mean values showed that all dimensions were higher for males compared to females. Mean measurements between males and females are shown in [Figure 3]. The F-statistic values indicated that mandibular measurements expressing the greatest sexual dimorphism were minimum ramus breadth followed by condylar height and projective height of ramus.
Figure 3: Mean measurements in males and females

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Table 1: Descriptive statistics

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The sex could be determined from calculations using the equations given below [Table 2]:

D Male : −185.622 + 1.361 (max. ramus breadth) + 1.087 (min. ramus breadth) + 2.253 (condylar height) − 0.717 (projective height of ramus) + 0.081 (coronoid height)

D Female : −161.761 + 1.276 (max. ramus breadth) + 0.948 (min. ramus breadth) + 2.220 (condylar height) − 0.753 (projective height of ramus) + 0.063 (coronoid height)
Table 2: Linear discriminant function

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For classifying a given sample as male or female, the higher/maximum value of the two equations is considered. With all the variables in consideration, 76% of the cases were classified correctly [Table 3] and the accuracy can be increased by repeated iterations. In this study, the sectioning point was found to be −0.667. Values greater than this sectioning point indicate male and values lesser than this point indicate female [Table 4].
Table 3: Prediction accuracy

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Table 4: Standardized and unstandardized coefficients in original samples

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   Discussion Top


The identification of sex from human remains is of fundamental importance in forensic medicine and anthropology, especially in criminal investigations as well as in the identification of missing persons and in attempts at reconstructing the lives of ancient populations. One of the important aspects of forensics is to determine sex from fragmented jaws and dentition. [7] Identification of sex based on morphological marks is subjective and likely to be inaccurate, but methods based on measurements and morphometry are accurate and can be used in determination of sex from the skull. [6],[11],[12] Mandibles were used for the analysis for two simple reasons: firstly, there appears to be a paucity of standards utilizing this element and secondly, this bone is often recovered largely intact. [5]

The accuracy of panoramic radiography in providing anatomic measurements has been established. Orthopantomograph has been advocated routinely and widely used by the clinicians as an appropriate screening tool for the diagnosis of oral diseases. The principal advantages of panoramic images are their broad coverage, low patient radiation dose, and the short time required for image acquisition. [13] Other advantages are that interference of superimposed images are not encountered. Also the contrast and brightness enhancement and enlargement of images provide an accurate and reproducible method of measuring the chosen points. [14],[15] The limitations of this technique are magnification and geometric distortion, the vertical dimension in contrast to the horizontal dimension is little altered, and this technique is quite sensitive to positioning errors because of relatively narrow image layer. [13] However, in our study, this limitation did not affect our results since all images were uniformly magnified.

Kambylafkas et al. [16] concluded that the use of the panoramic radiograph for evaluation of total ramal height is reliable and an asymmetry of more than 6% is an indication of a true asymmetry. Schulze et al.[9] found that the most reliable measurements were obtained for linear objects in the horizontal plane and digital measurements are sufficiently accurate for clinical use.

In this study, mandibular ramus measurements were subjected to discriminant function analysis. Each of the five variables measured on mandibular ramus using orthopantomograph showed statistically significant sex differences between sexes, indicating that ramus expresses strong sexual dimorphism. The mandibular ramus demonstrated greatest univariate sexual dimorphism in terms of minimum ramus breadth, condylar height, followed by projective height of ramus. Overall prediction rate using all five variables was 76% and the accuracy can be increased by repeated iterations.

Earliest studies on mandible by Morant et al. (1936), Martin (1936), and Hrdlicka (1940) (cited in Humphrey et al.) have established the usefulness of mandible for determination of sex. They found that the sexual differences were highest in height of the ramus, thus emphasizing that sex differences are more pronounced in mandibular ramus than in body.

Humphrey et al. [6] emphasized that almost any site of mandibular bone deposition, or resorption, or remodeling for that matter, seems to have a potential for becoming sexually dimorphic. Hence, mandibular condyle and ramus in particular are generally the most sexually dimorphic as they are the sites associated with the greatest morphological changes in size and remodeling during growth.

Various studies have investigated the sexual dimorphism of the mandibular ramus flexure using direct visual assessment, e.g. Donnelly et al. (1998), Indrayana et al. (1998), Hill (2000), and Kemkes-Grottenthaler et al. (2002), and found that results of these studies were contradictory and not repeatable. [17] Haun (2000) questioned the predictive accuracy of mandibular ramus flexure as a single indicator of sexual dimorphism and suggests that caution be used when applying this technique in the absence of other morphological and osteometric indicators, especially in the case of fragmentary forensic or rare fossil remains. [18]

Giles measured mandibles of known sex using anthropometric measurements and reported mandibular ramus height, maximum ramus breadth, and minimum ramus breadth as highly significant, with an accuracy of 85% in American Whites and Negroes. [19] Steyn and Iscan (1998) achieved an accuracy of 81.5% with five mandibular parameters (i.e. bigonial breadth, total mandibular length, bicondylar breadth, minimum ramus breadth, and gonion-gnathion) in South African Whites, which is comparable with the present study results. [1],[20] Dayal et al. (2008) found mandibular ramus height to be the best parameter in their study, with 75.8% accuracy. [1]

Saini et al. conducted a study on dry adult mandibles of northern part of India and found that ramus expresses strong sexual dimorphism in this population. The overall prediction rate using five variables was 80.2%. The best parameters were coronoid height, condylar height, and projective height of ramus, and breadth measurements were not very dimorphic in their sample. [1]

Minimum ramus breadth measurement was found to be the best parameter in the present study, which is consistent with other osteometric studies by Giles (1964) and Vodanovic (2006), where breadth measurements were found to be very dimorphic. This is related to the differences in musculoskeletal development and to the differences related to a different growth trajectory in males and females. [6],[19]

Limitations of this study are the inability to reliably assign sex in the sub-adult range and inability to assess the gender in case of edentulous patients.

It has been established that socio-environmental factors (e.g. nutrition, food, climate, pathologies, etc.) influence the development, and thus the appearance of bones. [1],[21] Numerous studies have demonstrated that skeletal characteristics differ in each population and have emphasized the need for population-specific osteometric standards for sex determination. [1],[7],[19]


   Conclusion Top


Mandibular ramus can be considered as a valuable tool in gender determination since it possesses resistance to damage and disintegration processes. We found that mandibular ramus measurements using orthopantomographs were reliable for sex determination. Hence, we strongly suggest the use of mandibular ramus as an aid for gender determination in forensic analysis. In view of these findings, further studies on more diverse populations to assess the significance of these parameters are recommended.

 
   References Top

1.Saini V, Srivastava R, Rai RK, Shamal SN, Singh TB, Tripathi SK. Mandibular ramus: An indicator for sex in fragmentary mandible. J Forensic Sci 2011;56(Suppl1):S13-6.  Back to cited text no. 1
    
2.Scheuer L. Application of osteology to forensic medicine. Clin Anat 2002;15:297-312.   Back to cited text no. 2
    
3.Duriæ M, Rakoceviæ Z, Doniæ D. The Reliability of sex determination of skeletons from forensic context in the Balkans. Forensic Sci Int 2005;147:159-64.  Back to cited text no. 3
    
4.Hu KS, Koh KS, Han SH, Shin KJ, Kim HJ. Sex determination using nonmetric characteristics of the mandible in Koreans. J Forensic Sci 2006;51:1376-82.  Back to cited text no. 4
    
5.Franklin D, O'Higgins P, Oxnard CE, Dadour I. Discriminant function sexing of the mandible of Indigenous South Africans. Forensic Sci Int 2008;179:84.e1-5.  Back to cited text no. 5
    
6.Humphrey LT, Dean MC, Stringer CB. Morphological variation in great ape and modern human mandibles. J Anat 1999;195:491-513.  Back to cited text no. 6
    
7.Vodanovic M, Dumancic J, Demo Z, Mihelic D. Determination of sex by discriminant function analysis of mandibles from two Croatian archaeological sites. Acta Stomatol Croat 2006;40:263-77.  Back to cited text no. 7
    
8.Sassouni V. Dentofacial radiography in forensic dentistry. J dent Res Jan./Feb. Supplement 1963;42(1):274-302  Back to cited text no. 8
    
9.Schulze R, Krummenauer F, Schalldac F, d'Hooedt B. Precision and accuracy of measurements in digital panoramic radiography. Dentomaxillofac Radiol 2000;29:52-6.  Back to cited text no. 9
    
10.Kahana T, Hiss J. Forensic radiology. Br J Radiol 1999;72:129-33.  Back to cited text no. 10
    
11.Franklin D, Oxnard CE, O'Higgins P, Dadour I. Sexual dimorphism in the subadult mandible: Quantification using geometric morphometrics. J Forensic Sci 2007;52:6-10.  Back to cited text no. 11
    
12.Franklin D, O'Higgins P, Oxnard CE. Sexual dimorphism in the mandible of indigenous South Africans: A geometric morphometric approach. South African Journal of Science 2008;104:101-6.  Back to cited text no. 12
    
13.Razi T, Moslemzade SH, Razi S. Comparison of linear dimensions and angular measurements on panoramic images taken with two machines. Journal of Dental Research, Dental Clinics, Dental Prospects 2009;3:7-10.  Back to cited text no. 13
    
14.Shahabi M, Ramazanzadeh BA, Mokhber N. Comparison between the external gonial angle in panoramic radiographs and lateral cephalograms of adult patients with Class I malocclusion. J Oral Sci 2009;51:425-9.  Back to cited text no. 14
    
15.Laster WS, Ludlow JB, Bailey LJ, Hershey HG. Accuracy of measurements of mandibular anatomy and prediction of asymmetry in panoramic radiographic images. Dentomaxillofac Radiol 2005;34:343-9.  Back to cited text no. 15
    
16.Kambylafkas P, Murdock E, Gilda E, Tallents RH, KyrkanidesS. Validity of panoramic radiographs for measuring mandibular asymmetry. Angle Orthod 2006;76:388-93.  Back to cited text no. 16
    
17.Oettlé AC, Pretorius E, Steyn M. Geometric Morphometric Analysis of Mandibular Ramus Flexure. Am J Phys Anthropol 2005;128:623-9.  Back to cited text no. 17
    
18.Hill CA. Technical Note: Evaluating Mandibular Ramus Flexure as a Morphological Indicator of Sex. Am J Phys Anthropol 2000;111:573-7.  Back to cited text no. 18
    
19.Giles E. Sex determination by discriminant function analysis of the mandible. Am J Phys Anthropol 1964;22:129-35.  Back to cited text no. 19
    
20.Steyn M, Iscan MY. Sexual dimorphism in the crania and mandibles of South African whites. Forensic Sci Int 1998;98:9-16.  Back to cited text no. 20
    
21.Suazo GI, Zavando MD, Smith RL. Evaluating accuracy and precision in morphologic traits for sexual dimorphism in malnutrition human skull: A comparative study. Int J Morphol 2008;26:877-81.  Back to cited text no. 21
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]


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