Journal of Forensic Dental Sciences
IAFO CONFERENCE 2018 GOA - http://iafo2018goa.com/ Users Online: 587 
Home Print this page  Email this page Small font size Default font size Increase font size
Wide layoutNarrow layoutFull screen layout
  Home | About JFDS | Editorial Board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Online submission | Contact us | Advertise | Login 
ORIGINAL ARTICLE
Year : 2013  |  Volume : 5  |  Issue : 1  |  Page : 42-46

Assessment of morphological changes and DNA quantification: An in vitro study on acid-immersed teeth


1 Department of Oral and Maxillofacial Pathology, People's College of Dental Sciences and Research Centre, Bhopal (MP), India
2 Department of Oral and Maxillofacial Pathology, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India

Correspondence Address:
K Sowmya
Department of Oral and Maxillofacial Pathology, Peoples College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-1475.114560

Rights and Permissions

Context: Acid immersion of victim's body is one of the methods employed to subvert identification of the victim, and hence of the perpetrator. Being hardest and chemically the most stable tissue in the body, teeth can be an important forensic investigative medium in both living and nonliving populations. Teeth are also good reservoirs of both cellular and mitochondrial DNA; however, the quality and quantity of DNA obtained varies according to the environment the tooth has been subjected to. DNA extraction from acid-treated teeth has seldom been reported. Aims: The objectives of the present study were to assess the morphological changes along with DNA recovery from acid-immersed teeth. Materials and Methods: Concentrated hydrochloric acid, nitric acid, and sulfuric acid were employed for tooth decalcification. DNA was extracted on an hourly basis using phenol-chloroform method. Quantification of extracted DNA was done using a spectrophotometer. Results: Results showed that hydrochloric acid had more destructive capacity compared to other acids. Conclusion: Sufficient quantity of DNA was obtainable till the first 2 hours of acid immersion and there was an inverse proportional relation between mean absorbance ratio and quantity of obtained DNA on an hourly basis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2201    
    Printed86    
    Emailed0    
    PDF Downloaded298    
    Comments [Add]    
    Cited by others 1    

Recommend this journal