Journal of Forensic Dental Sciences

ORIGINAL ARTICLE
Year
: 2010  |  Volume : 2  |  Issue : 1  |  Page : 32--36

Isolation of epithelial cells from acrylic removable dentures and gender identification by amplification of SRY gene using real time PCR


Renjith George, G Sriram, TR Saraswathi, B Sivapathasundharam 
 Department of Oral Pathology, Meenakshi Ammal Dental College, Chennai, India

Correspondence Address:
B Sivapathasundharam
Professor and Head, Department of Oral Pathology, Meenakshi Ammal Dental College, Alappakkam Main Road, Madhuravoyal, Chennai - 600 095
India

This study evaluates the usefulness of acrylic dentures as the source of DNA for forensic analysis. Thirty-eight samples (21 males and 17 females) were collected and stored for different time periods. The epithelial cells adhered to the dentures were retrieved and the genomic DNA was extracted. All the samples yielded sufficient amount of DNA for analysis irrespective of the storage time. Gender determination was done by amplification of the sex determining region on the Y chromosome (SRY) using real-time polymerase chain reaction with 100% accuracy, within minimal time. With this study, we conclude that saliva-stained acrylic dentures can act as a source of forensic DNA and co-amplification of SRY gene with other routine sex typing markers will give unambiguous gender identification.


How to cite this article:
George R, Sriram G, Saraswathi T R, Sivapathasundharam B. Isolation of epithelial cells from acrylic removable dentures and gender identification by amplification of SRY gene using real time PCR.J Forensic Dent Sci 2010;2:32-36


How to cite this URL:
George R, Sriram G, Saraswathi T R, Sivapathasundharam B. Isolation of epithelial cells from acrylic removable dentures and gender identification by amplification of SRY gene using real time PCR. J Forensic Dent Sci [serial online] 2010 [cited 2019 Dec 13 ];2:32-36
Available from: http://www.jfds.org/article.asp?issn=0975-1475;year=2010;volume=2;issue=1;spage=32;epage=36;aulast=George;type=0