Journal of Forensic Dental Sciences

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 8  |  Issue : 2  |  Page : 112--113

Evaluation of skeletal maturation using mandibular third molar development in Indian adolescents


Nishit Mehta1, Dolly Patel2, Falguni Mehta3, Bhaskar Gupta4, Grishma Zaveri5, Unnati Shah6,  
1 Department of Orthodontics, Government Dental College, Ahmedabad, Gujarat, India
2 Department of Orthodontics, AMC Dental College and Hospital, Ahmedabad, Gujarat, India
3 Department of Orthodontics, Government Dental College and Hospital, Ahmedabad, Gujarat, India
4 Department of Orthodontics, Jaipur Dental College and Hospital, Jaipur, Rajasthan, India
5 DDS Student, University of Pacific, Arthur A Dugoni School of Dentistry, San Francisco, USA
6 DDS Student, UCLA School of Dentistry, Los Angeles, USA

Correspondence Address:
Dr. Nishit Mehta
1, Gyandip Society, Dhumketu Road, Paldi, Ahmedabad - 380 007, Gujarat
India

Abstract

Objective: This study was done with the following objectives: to estimate dental maturity using the Demirjian Index (DI) for the mandibular third molar; to investigate the relationship between dental maturity and skeletal maturity among growing patients; to evaluate the use of the mandibular third molar as an adjunctive tool for adolescent growth assessment in combination with the cervical vertebrae; to evaluate the clinical value of the third molar as a growth evaluation index.Materials and Methods: Samples were derived from panoramic radiographs and lateral cephalograms of 615 subjects (300 males and 315 females) of ages ranging 9-18 years, and estimates of dental maturity (DI) and skeletal maturity [cervical vertebrae maturation indicators (CVMI)] were made.Results: A highly significant association (r = 0.81 for males and r = 0.72 for females) was found between DI and CVMI. DI Stage B corresponded to Stage 2 of CVMI (prepeak of pubertal growth spurt) in both sexes. In males, DI stages C and D represent the peak of the pubertal growth spurt. In females, stages B and C show that the peak of the pubertal growth spurt has not been passed. DI stage E in females and DI Stage F in males correlate that the peak of the pubertal growth spurt has been passed. Conclusion: A highly significant association exists between DI and CVMI. Mandibular third molar DI stages are reliable adjunctive indicators of skeletal maturity.



How to cite this article:
Mehta N, Patel D, Mehta F, Gupta B, Zaveri G, Shah U. Evaluation of skeletal maturation using mandibular third molar development in Indian adolescents.J Forensic Dent Sci 2016;8:112-113


How to cite this URL:
Mehta N, Patel D, Mehta F, Gupta B, Zaveri G, Shah U. Evaluation of skeletal maturation using mandibular third molar development in Indian adolescents. J Forensic Dent Sci [serial online] 2016 [cited 2019 Nov 14 ];8:112-113
Available from: http://www.jfds.org/text.asp?2016/8/2/112/186372


Full Text

 Introduction



The optimal timing for treatment in dentofacial orthopedics is linked intimately to the identification of periods of accelerated or intense growth that contribute significantly to the correction of skeletal imbalances in an individual patient. Precise evaluation of the developmental stage forms an integral part of both diagnosis and treatment plan for pediatric patients.[1] Different authors have advocated various methods of determining skeletal maturity based on radiographs of specific structures, such as epiphysis-diaphysis fusion of long bones,[2],[3] medial extremity of the clavicle,[4] epiphyseal head of the first rib,[5] epiphyseal union of the anterior iliac crest,[6] and fusion of the sphenoid bone with the basilar part of the occipital bone.[7],[8],[9] However, these skeletal methods not only present some inconveniences in view of the variability of bone maturation, as influenced by environmental factors,[10] but necessitate additional radiographs as well. The hand-wrist radiograph has been one of the most commonly used methods for skeletal developmental assessment.[11]

Panoramic and lateral cephalometric radiographs form a part of essential diagnostic examination for the comprehensive diagnosis and treatment planning of an orthodontic patient. Apart from its use in studying skeletal, dental, and soft-tissue structures, the usefulness of lateral cephalometric radiographs for assessing maturation has also been studied.[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24] A distinct advantage of cervical maturity evaluation is that it does not entail extra radiation exposure for the orthodontic patient. Hand-wrist and cervical maturation methods are highly correlated, therefore it does not justify to take an extra hand wrist radiograph for skeletal maturation determination.[15],[16],[17],[18],[23] Hassel and Farman [12] suggested six stages of classification of the cervical vertebrae based on the shape of the second to the fourth vertebrae on lateral cephalogram.

Apart from viewing maxillomandibular structures, an orthopantomogram can also be used to assess the dental maturity of the patient. Dental development can be assessed from either the phase of tooth eruption or the stage of tooth calcification, with the latter being more reliable.[25],[26] Demirjian et al.[26] proposed a method for estimation of the subject's dental maturity based on eight calcification stages, which span from the first sign of tooth calcification to apex closure for the seven left permanent mandibular teeth. However, short time intervals between different developmental stages may adversely affect the possibility of identifying the relationship between tooth development and maturation.[27] The third molar offers a unique advantage over other teeth because its development tends to continue for a longer period and until a later age (8-18 years). Most patients undergoing orthodontic treatment belong to this group. The continuation of third molar development during adolescence provides a different point of reference from the other teeth. If a positive correlation between third molar development and general growth can be evaluated, it would be possible to use the third molar as a growth indicator in pubertal patients.

This study has been conducted to estimate dental maturity using the Demirjian Index (DI) for the mandibular third molar, to investigate the relationships between dental maturity and cervical vertebral skeletal maturity among growing patients, and to evaluate the clinical value of the third molar as a growth evaluation index.

 Aims and Objectives



The aims and objectives of this study were:

To estimate dental maturity using the DI for the mandibular third molarTo investigate the relationship between dental maturity and skeletal maturity among growing patientsTo evaluate the use of the mandibular third molar as an adjunctive tool for assessment of adolescent growth in combination with cervical vertebraeTo evaluate the clinical value of the third molar as a growth evaluation index.

 Materials and Methods



The samples were derived from panoramic, lateral cephalometric radiographs of 300 male and 315 female subjects registered as patients at the Department of Orthodontics at Government Dental College and Hospital, Ahmedabad, Gujarat, India. The age range of the sample in females was 9-18 years with the mean age of 14 years, and in males was 10-18 years with the mean age of 13.4 years.

Selection criteria

The following criteria in individuals were used for the selection of subjects:

Well-nourished and free of any known serious diseaseNormal growth and dental development; no missing teeth or supernumerary teeth No congenital oral or maxillofacial anomalies such as cleft lip and palate.

Dental maturity evaluation on panoramic radiograph

In this study, the lower left third molar has been used as a sample because of higher estimation errors in calculating the maturation of the upper molar than the lower molar. The root of the upper third molar may overlap with anatomic structures such as the palate, the inferior border of the zygomatic arch, or the maxillary sinus septum. Therefore, it can be difficult to observe the root. Cases in which the development of the left and right third molars differed remarkably or in which developmental anomalies were observed were excluded. Tooth calcification was rated according to the method described by Demirjian et al.,[26] in which one of eight stages of calcification (A to H) was assigned to the third molar tooth [Table 1] and [Figure 1].{Table 1}{Figure 1}

Cervical vertebrae maturity evaluation on lateral cephalogram

The cervical vertebrae maturation indicators (CVMI) were evaluated by dividing the second, third, and fourth vertebrae into six groups depending on their maturation patterns on lateral cephalogram using the classification of Hassel and Farman [12] [Table 2] and [Figure 2].{Table 2}{Figure 2}

Each sample is assigned DI (A to H) based on the mandibular third molar development and cervical maturation stage based on skeletal maturation.

All the data thus compiled from the lateral cephalogram and panoramic radiographs of the samples were subjected to appropriate statistical analysis.

Statistical analysis

We performed statistical analysis using Microsoft Office Excel 2007 (Microsoft) and IBM - SPSS version 22 software (IBM Inc.). The mean values of chronologic age in males and females were calculated at each stage of the DI. The median values of CVMI stages were calculated at each stage of the DI in males and females. To study the relationship between DI and the CVMI, the frequency and the percentage distribution of the stages of calcification were recorded for each tooth, and these were calculated separately for male and female subjects. Cross-tabular statistics were performed. The Pearson Chi-square test (x 2) value and correlation coefficient (r) were estimated to determine the relationships between DI and CVMI. Independent sample t-tests were performed for comparison of mean age at different stages of DI between males and females.

 Results



[Table 3] and [Table 4] show the mean age for each stage of DI in males and females, respectively. Although the mean ages at different stages of DI individually indicate that lower third molar development is completed earlier in males than in females, it was statistically insignificant except in Stage D [Table 5].{Table 3}{Table 4}{Table 5}

In this study, the results of the Chi-square test show the statistically significant interdependence of DI and CVMI in males (P < 0.0001) [degree of freedom (df) =35] [Table 6] as well as in females (P < 0.0001) (df = 28) [Table 7].{Table 6}{Table 7}

There was found a statistically significant positive correlation between the development of the third molar (DI) and CVMI in both males (r = 0.81) (P < 0.0001) and females (r = 0.72) (P < 0.0001). DI and mean age also have a high positive correlation in both males (r = 0.79) (P < 0.0001) and females (r = 0.80) (P < 0.0001). In addition, CVMI and mean age have a high positive correlation in both males (r = 0.84) (P < 0.0001) and females (r = 0.84) (P < 0.0001) [Table 8] and [Table 9].{Table 8}{Table 9}

In males, distribution of CVMI to DI [Table 10] shows that DI Stage B is characterized by their concentration of CVMI Stage 2 (66.7%), DI Stage C is characterized by their concentration of CVMI Stage 3 (60.0%), and DI Stage D is characterized by their concentration of CVMI Stage 3 (40.9%) and Stage 4 (50%). In females, distribution of CVMI stage to DI [Table 11] shows that DI Stage B is characterized by their concentration of CVMI Stage 2 (42.9%) and Stage 3 (42.9%), DI Stage C is characterized by their concentration of CVMI Stage 3 (53.3%) and Stage 4 (33.3%), DI Stage D is characterized by their concentration of CVMI Stage 4 (31.8%) and Stage 5 (43.2%), and DI Stage E is characterized by their concentration of CVMI Stage 5 (42.9%) and Stage 6 (42.9%).{Table 10}{Table 11}

 Discussion



Maturational indices have been proposed to evaluate skeletal maturity in the growing patient when planning orthodontic/orthopedic treatment or for clinical and research purposes. The ease of recognizing the stages of dental development and the availability of panoramic radiography are practical reasons for attempting to assess physiologic maturity without resorting to hand-wrist radiograph. The “As Low As Reasonably Achievable” (ALARA) principle is especially important for children and young adults, and thus high-radiation methods should not be used frequently to assess growth.

Dental maturity, in particular, has an advantage of being easily evaluable during regular dental treatment.[28] Dental maturity is determined by the stage of tooth eruption or the stage of tooth formation. Chertkow [29] indicated that the completion of root formation in the mandibular canines prior to apical closure may be used clinically as a maturity indicator for pubertal growth spurts with the same degree of confidence as the indicators described for hand-wrist radiographs among white children. However, root formation and apex closure of mandibular canines are completed by the age of 13 years, and most children exhibit active growth up to the age of 16-17 years.[30] A study by Bolanos et al.[31] showed that the root formation of the third molar is complete at an average of 18.5 years. The third molar has a unique feature compared to other teeth in that its development tends to continue over a long period and until a later age. The continuation of third molar development during adolescence provides a different point of reference from the other teeth.[28] Recent studies have verified that Demirjian's classification system shows the least intraexaminer and interexaminer errors and a high correlation with biological age.[28] Therefore, dental maturity in this study was determined by evaluating the stages of tooth formation of the third molar by Demirjian's method.

The results also show a statistically significant positive correlation between dental maturity (DI) and skeletal maturity (CVMI) consistent with the findings of Engstrom et al.,[27] Krailassiri et al.,[11] Uysal et al.,[32] and Kalinowska et al.,[1] who also have suggested a strong relationship between dental maturity and skeletal maturity.

For both sexes, DI Stage B showed the highest percentage distribution at Stage 2 of CVMI [Table 10] and [Table 11]. The relationship between skeletal maturity and peak height velocity (PHV) is well established.[33],[34],[35] Fishman [33] and Hagg and Taranger [34] found that the appearance of the adductor sesamoid of the thumb indicates the beginning of the pubertal growth spurt (onset of PHV), which corresponds to Stage 2 of CVMI.[24] In the present study, Stage 2 of CVMI corresponded with DI Stage B in both males and females. Hence, DI Stage B signifies the prepeak of the pubertal growth spurt or onset of PHV in both the sexes.

In the present study, for male subjects, stages C and D corresponded to CVMI Stage 3 [Table 10], while for female subjects, stages B and C corresponded to CVMI Stage 3 [Table 11]. Bjork and Helm [35] found that the MP3cap stage heralds the peak of the pubertal growth spurt, which corresponds to Fishman's [33] skeletal maturity indicator 6 (stage 3 of the CVMI [24]). It can be inferred that DI stages C and D in males represent the peak of the pubertal growth spurt, while DI stages B and C in females show that the peak of the pubertal growth spurt has not been passed.

In this study in males, the samples in Demirjian stages E or above are predicted to occur at the stages higher than CVMI Stage 3, and the samples in Demirjian stages F are predicted to occur at the stages higher than CVMI Stage 4 [Table 10]. In females, the samples in Demirjian stages E or above are predicted to occur at stages higher than CVMI Stage 4 [Table 11]. In contrast to the study by Sun-Mi Cho et al.[28] where it was shown that in females, the samples in Demirjian stages E or above are predicted to occur at the higher stages of CVMI Stage 5. Thus it can be concluded that in females DI Stage E and in males DI Stage F correlate that the peak of the pubertal growth spurt has been passed.

The mean ages at different stages of DI individually indicate that lower third molar development is complete earlier in males than in females, but it is statistically insignificant except for Stage D, which signifies the peak of the pubertal growth spurt in males. This finding is consistent with the results of Bolanos et al.,[31] which showed for males and females similar age distribution, mean age, and developmental stages, with negligible differences in the mean degree of formation of the four molars. However, findings by Mincer et al.,[36] Solari et al.,[37] Sisman et al.,[38] and Golovcencu et al.[39] showed that lower third molar development is complete earlier in males than in females.

The revealed correlation between lower third molar development and skeletal maturity in this study will allow clinicians to use the mandibular third molar as an adjunctive tool to assess adolescent growth, in combination with cervical vertebrae evaluations. Individual variations should be taken into consideration when using the developmental stage of the third molar in growth evaluations, as third molars are known for their many variations, based on previous studies. This cross-sectional study has limitations in terms of the evaluation of results because the subjects in this study were in the pubertal growth period and mainly concentrated in DI stages C, D, and E. Increasing the number of subjects in each stage would further increase the accuracy of results obtained. Further longitudinal study with a larger sample size is recommended for establishing specific guidelines to use the DI of the mandibular third molar for growth assessment.

 Conclusion



Both males and females show statistically significant positive correlation between the development of the third molar (DI) and CVMIDI stage B signifies the prepeak of pubertal growth spurt in both males and femalesIn males, DI stages C and D represent the peak of the pubertal growth spurtIn females, stages B and C correlate that the peak of the pubertal growth spurt has not been passedDI Stage E in females and DI Stage F in males correlate that the peak of the pubertal growth spurt has been passedBoth sexes present similar developmental pace of the third molar, except for DI Stage D in males that signifies the peak of puberty.

Dental maturity evaluation using the mandibular third molar can be used as an adjunctive tool for adolescent growth assessment in clinical orthodontics.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

References

1Różyło-Kalinowska I, Kolasa--Rączka A, Kalinowski P. Relationship between dental age according to Demirjian and cervical vertebrae maturity in Polish children. Eur J Orthod 2011;33:75-83.
2Greulich WW, Pyle SI. Radiographic Atlas of Skeletal Development of the Hand and Wrist. 2nd ed. Stanford: Stanford University Press; 1959.
3Banerjee KK, Agarwal BB. Estimation of age from epiphyseal union at the wrist and ankle joints in the capital city of India. Forensic Sci Int 1998;98:31-9.
4Kreitner KF, Schweden FJ, Riepert T, Nafe B, Thelen M. Bone age determination based on the study of the medial extremity of the clavicle. Eur Radiol 1998;8:1116-22.
5Kunos CA, Simpson SW, Russell KF, Hershkovitz I. First rib metamorphosis: Its possible utility for human age-at-death estimation. Am J Phys Anthropol 1999;110:303-23.
6Webb PA, Suchey JM. Epiphyseal union of the anterior iliac crest and medial clavicle in a modern multiracial sample of Americans males and females. Am J Phys Anthropol 1985;68:457-66.
7Coben SE. The spheno-occipital synchondrosis: The missing link between the profession's concept of craniofacial growth and orthodontic treatment. Am J Orthod Dentofacial Orthop 1998;114:709-14.
8Meneses M, Laude M, Casero LG. Age of closure of the spheno-occipital junction of the clivus, Determination by MRI. Bull Assoc Anat (Nancy) 1994;78:27-9.
9Sahni D, Jit I, Neelam S, Suri S. Time of fusion of the basisphenoid with the basilar part of the occipital bone in northwest Indian subjects. Forensic Sci Int 1998;98:41-5.
10Chaillet N, Willems G, Demirjian A. Dental maturity in Belgian children using Demirjian's method and polynomial functions: New standard curves for forensic and clinical use. J Forensic Odontostomatol 2004;22:18-27.
11Krailassiri S, Anuwongnukroh N, Dechkunakorn S. Relationships between dental calcification stages and skeletal maturity indicators in Thai Individuals. Angle Orthod 2002;72:156-66.
12Hassel B, Farman AG. Skeletal maturation evaluation using cervical vertebrae. Am J Orthod Dentofacial Orthop 1995;107:58-66.
13García-Fernandes P, Torre H, Flores L, Rea J. The cervical vertebrae as maturational indicators. J Clin Orthod 1998;32:221-5.
14Baccetti T, Franchi L, McNamara JA Jr. An improved version of the cervical vertebral maturation (CVM) method for the assessment of mandibular growth. Angle Orthod 2002;72:316-23.
15Mito T, Sato K, Mitani H. Cervical vertebral bone age in girls. Am J Orthod Dentofacial Orthop 2002;122:380-5.
16Flores-Mir C, Burgess CA, Champney M, Jensen RJ, Pitcher MR, Major PW. Correlation of skeletal maturation stages determined by cervical vertebrae and hand-wrist evaluations. Angle Orthod 2006;76:1-5.
17Soegiharto BM, Moles DR, Cunningham SJ. Discriminatory ability of the skeletal maturation index and the cervical vertebrae maturation index in detecting peak pubertal growth in Indonesian and white subjects with receiver operating characteristics analysis. Am J Orthod Dentofacial Orthop 2008;134:227-37.
18San Román P, Palma JC, Oteo MD, Nevado E. Skeletal maturation determined by skeletal vertebrae development. Eur J Orthod 2002;24:303-11.
19Gandini P, Mancini M, Andreani F. A comparison of hand-wrist bone and cervical vertebral analyses in measuring skeletal maturation. Angle Orthod 2006;76:984-9.
20Alkhal HA, Wong RW, Rabie AB. Correlation between chronological age, cervical vertebral maturation and Fishman's skeletal maturity indicators in southern Chinese. Angle Orthod 2008;78:591-6.
21Santiago RC, da Miranda Costa LF, Vitral RW, Fraga MR, Bolognese AM, Maia LC. Cervical vertebral maturation as a biologic indicator of skeletal maturity. Angle Orthod 2012;82:11123-31.
22Lamparski D. Skeletal Age Assessment Utilizing Cervical Vertebrae. Pittsburgh: University of Pittsburgh; 1972.
23Rasool G, Bashir U, Kundi IU. Comparative evaluation between cervical vertebrae and hand-wrist maturation for assessment of skeletal maturity in orthodontic patients. Pak Oral Dental J 2010;30:85-95.
24Kamal M, Ragini, Goyal S. Comparative evaluation of hand wrist radiographs with cervical vertebrae for skeletal maturation in 10-12 years old children. J Indian Soc Pedod Prev Dent 2006;24:124-35.
25Nolla CM. The development of the permanent teeth. J Dent Child 1960;27:254-63.
26Demirjian A, Goldstein H, Tanner JM. A new system of dental age assessment. Hum Biol 1973;45:211-27.
27Engström C, Engström H, Sagne S. Lower third molar development in relation to skeletal maturity and chronological age. Angle Orthod 1983;53:97-106.
28Cho SM, Hwang CJ. Skeletal maturation evaluation using mandibular third molar development in adolescents. Korean J Orthod 2009;39:120-9.
29Chertkow S. Tooth mineralization as an indication of the pubertal growth spurt. Am J Orthod 1980;77:79-91.
30Kumar S, Singla A, Sharma R, Virdi M, Anupam A, Mittal B. Skeletal maturation evaluation using mandibular second molar calcification stages. Angle Orthod 2012;82:501-6.
31Bolanos MV, Moussa H, Manrique MC, Bolaños MJ. Radiographic evaluation of third molar development in Spanish children and young people. Forensic Sci Int 2003;133:212-9.
32Uysal T, Sari Z, Ramoglu SI, Basciftci FA. Relationships between dental and skeletal maturity in Turkish subjects. Angle Orthod 2004;74:657-64.
33Fishman LS. Radiographic evaluation of skeletal maturation. A clinically oriented method based on hand-wrist films. Angle Orthod 1982;52:88-112.
34Hagg U, Taranger J. Maturation indicators and the pubertal growth spurt. Am J Orthod 1982;82:299-309.
35Björk A, Helm S. Prediction of the age of maximum pubertal growth in body height. Angle Orthod 1967;37:134-43.
36Mincer HH, Harris EF, Berryman HE. The A.B.F.O. study of third molar development and its use as an estimator of chronological age. J Forensic Sci 1993;38:379-90.
37Solari AC, Abramovitch K. The accuracy and precision of third molar development as an indicator of chronological age in Hispanics. J Forensic Sci 2002;47:531-5.
38Sisman Y, Uysal T, Yagmur F, Ramoglu SI. Third-molar development in relation to chronologic age in Turkish children and young adults. Angle Orthod 2007;77:1041-6.
39Golovcencu L, Scripcaru C, Zegan G. Third molar development in relation to chronological age in Romanian children and young adults. Rom J Leg Med 2009;17:277-82.