DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR
##plugins.themes.academic_pro.article.main##
Abstract
Background: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine. Objective: To isolate the DNA from dental hard tissue (enamel and dentin) from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex using polymerized chain reactor (PCR). Materials and Methods: DNA was extracted following phenol/chloroform (organic) extraction from 20 male and 20 female teeth. The samples that contain the amelogenin gene (amel) were amplified by PCR. The products of the PCR were run on agarose gel with ethidium bromide staining on gel documentation system. Results: The results on the gel showed the presence of X-specific bands at 212 bp and Y-specific bands at 218 bp. Males were distinguished from females by the presence of two bands whereas female samples showed only one, that is, X-specific band on the gel. The gender from the known samples was determined with complete accuracy, and the results were analyzed statistically by the Chi-square test. Conclusion: In our study, the PCR-based method showed 100% specificity and sensitivity.
##plugins.themes.academic_pro.article.details##
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
Subramanian Praveen Kumar, & Nalini Aswath. (2016). DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR. Journal of Forensic Dental Sciences, 8(1), 18–21. https://doi.org/10.4103/0975-1475.176953
References
- Pretty IA, Sweet D. A look at forensic dentistry-Part 1: The role of teeth in the determination of human identity. Br Dent J 2001;190:359‑66.
- Wilson DF, Brown KA. ‘Forensic Odontology’ Essentials of Oral Health. San Diego: Viva Publication; 1997. p. 752-3.
- Simmer JP, Hu JC. Dental enamel formation and its impact on clinical dentistry. J Dent Educ 2001;65:896‑905.
- GharibAM. Use of teeth samples for gender determination by PCR in Iraqi case. Iraqi J Biotech 2010;9:819-27.
- Sivagami AV, Rao AR, Varshney U. A simple and cost‑effective method for preparing DNA from the hard tooth tissue, and its use in polymerase chain reaction amplification of amelogenin segment for sex determination in an Indian population. Forensic Sci Int 2000;110:107-15.
- GaytmennR, Sweet D. Quantification of forensic DNA from various regions of human teeth. J Forensic Sci 2003;48:622‑5.
- Santo AR, Line S. The enamel organic matrix: Structure and function. Braz J Oral Sci 2005;4:716‑24.
- Bansal AK, Shetty DC, Bindal R, Pathak A. Amelogenin: A novel protein with diverse applications in genetic and molecular profiling. J Oral Maxillofac Pathol 2012;16:395‑9.
- Rao S. Polymerase Chain Reaction. Department of Microbiology. Davangree: JJMC; 2006. p. 1‑3.
- Kramvis A, Bukofzer S, Kew MC. Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit, GeneReleaser, and the phenol-chloroform method. J Clin Microbiol 1996;34:2731‑3.