Renjith George; G Sriram; T Saraswathi; B Sivapathasundharam

doi:10.4103/0974-2948.71055

Authors

Renjith George Department of Oral Pathology, Meenakshi Ammal Dental College, Chennai
G Sriram Department of Oral Pathology, Meenakshi Ammal Dental College, Chennai
T Saraswathi Department of Oral Pathology, Meenakshi Ammal Dental College, Chennai
B Sivapathasundharam Department of Oral Pathology, Meenakshi Ammal Dental College, Chennai

DOI:

https://doi.org/10.4103/0974-2948.71055

Keywords:

Acrylic removable dentures, gender identification, real-time PCR, SRY

Abstract

This study evaluates the usefulness of acrylic dentures as the source of DNA for forensic analysis. Thirty-eight samples (21 males and 17 females) were collected and stored for different time periods. The epithelial cells adhered to the dentures were retrieved and the genomic DNA was extracted. All the samples yielded sufficient amount of DNA for analysis irrespective of the storage time. Gender determination was done by amplification of the sex determining region on the Y chromosome (SRY) using real-time polymerase chain reaction with 100% accuracy, within minimal time. With this study, we conclude that saliva-stained acrylic dentures can act as a source of forensic DNA and co-amplification of SRY gene with other routine sex typing markers will give unambiguous gender identification.

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References

Inoue M, Hanaoka Y, Minaguchi K. Personal identification by DNA analysis of samples from dental prostheses made of acrylic resin. Bull Tokyo Dent Coll 2000;41:175-85.

Bazzi MD. DNA quantification. Available from: http://faculty.ksu. edu.sa/Bazzi-MD/LBCH435/12-DNA%20Quantitation.doc. [Last cited on 2009 Nov 24].

Rajendran R, Sivapathasundharam B. Shafer’s text book of oral pathology. 6th ed. New Delhi: Elsevier; 2009.

Das N, Gorea RK, Gargi J, Singh JR. Sex determination from pulpal tissue. J Indian Forensic Med 2004;26:50-4.

Tanaka M, Yoshimoto T, Nozawa H, Ohtaki H, Kato Y, Sato K,et al. Usefulness of a toothbrush as a source of evidential DNA for typing. J Forensic Sci 2000;45:674-6.

Hochmeister MN, Budowle B, Jung J, Borer UV, Comey CT, et al. PCR-based typing of DNA extracted from cigarette butts. Int J Legal Med 1991;104:229-33.

Sinclair K, McKechnie VM, DNA extraction from stamps and envelope flaps using QIAamp and QIAshredder. J Forensic Sci 2000;45:229-30.

Lorente M, Entrala C, Lorente JA, Alvarez JC, Villanueva E, Budowle B. Dandruff as a potential source of DNA in forensic casework. J Forensic Sci 1998;43:901-2.

Sinha SK, Hedges DJ, Batzer MA. Assay for human DNA for gender determination. 2005. Available from: http://www.freepatentsonline. com/y2005/0069903.html. [Last cited on 2009 Nov 24].

Genetic Home Reference. Available from: http://ghr.nlm.nih.gov/ gene=sry. [Last cited on 2009 Nov 17].

Drobnic K. A new primer set in a SRY gene for sex identification. Int Congr Ser 2006;1288:268-70.

Kastelic V, Budowle B, Drobnic K. Validation of SRY marker for forensic casework analysis. J Forensic Sci 2009;54:551-5.

Thangaraj K, Reddy AG, Singh L. Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? Int J Legal Med 2002;116:121-3.

Naito E, Dewa K, Yamanouchi H, Kominami R. Sex typing of forensic DNA samples using male- and female-specific probes. J Forensic Sci 1994;39:1009-17.

Steinlechner M, Berger B, Niederstätter H, Parson W. Rare failures in the amelogenin sex test. Int J Legal Med 2002;116:117-20.

Ghosh S. Sexuality, Gender Identity. Available from: http:// emedicine.medscape.com/article/917990-overview. [Last cited on 2009 Nov 24].

Dauwerse JG, Hansson KB, Brouwers AA, Peters DJ, Breuning MH. An XX male with the sex-determining region Y gene inserted in the long arm of chromosome 16. Fertil Steril 2006; 86:463.e1-5.

Hong YC, Liu HM, Chen PS, Chen YJ, Lyou JY, Hu HY, et al. Hair follicle: A reliable source of recipient origin after allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant 2007;40:871-4.

von Wurmb-Schwark N, Bosinski H, Ritz-Timme S. What do the X and Y chromosomes tell us about sex and gender in forensic case analysis? J Forensic Leg Med 2007;14:27-30.

Dauber EM, Dorner G, Mitterbauer M, Wenda S, Faé I, Mayr WR. Discrepant results of samples taken from different tissues of a single individual. In: Bordeaux, Arcachon, editors. France: Oral presentation (O22) on the International Society for Forensic Genetics; 2003. p. 9-13

Cohena Y, Nagler A. Hematopoietic stem-cell transplantation using umbilical-cord blood. Leuk Lymphoma 2003;44:1287-99.

Bianchi DW. Circulating fetal DNA: Its origin and diagnostic potential-a review. Placenta 2004;25:S93-101.

Bayes-Genis A, Bellosillo B, de la Calle O, Salido M, Roura S, Ristol FS, et al. Identification of male cardiomyocytes of extracardiac origin in the hearts of women with male progeny: Male fetal cell microchimerism of the heart. J Heart Lung Transplant 2005;24:2179-83.

Costa JM, Benachi A, Gautier E, Jouannic JM, Ernault P, Dumez Y. First-trimester fetal sex determination in maternal serum using real-time PCR. Prenat Diagn 2001;21:1070-4.

Rao L, Suryanarayana V, Kanakavalli M, Padmalatha V, Raseswari T, Pratibha N, et al. Y chromosome microchimerism in female peripheral blood. Reprod Biomed Online 2008;17:575-8.

Klintschar M, Schwaiger P, Regauer S, Mannweiler S, Kleiber M. Persisting fetal microchimerism does not interfere with forensic Y-chromosome typing. Forensic Sci Int 2004;139:151-4.

Ajay P, Sivapathasundharam B, Sriram G, Rao GV. Sex determination using AMEL gene and personal identification using HLA-DQα from DNA isolated from dental pulp. J Forensic Odontol 2008;1:26-9.

Chang YM, Burgoyne LA, Both K. Higher failures of amelogenin sex test in an Indian population group. J Forensic Sci 2003;48:1309-13.

Kao LG, Tsai LC, Lee JC, Hsieh HM. Controversial cases of human gender identification by amelogenin test. Forensic Sci J 2007;6:6971.

Kashyap VK, Sahoo S, Sitalaximi T, Trivedi R. Deletions in the Y-derived amelogenin gene fragment in the Indian population. BMC Med Genet 2006;7:37